Title: Engineering angiogenesis following spinal cord injury: building Functional, stable blood vessels and promoting the formation of the blood-spinal cord barrier
Speaker: Erin Lavik, Sc.D. Seminar Series: Neural Prosthesis Seminar Date: September 12, 2008 Location: Nord 310 A&B Case Western Reserve University Sponsored By: The FES and the APT Centers
Presented By: Erin Lavik, Sc.D. Associate Professor of Biomedical Engineering School of Engineering and Applied Science Yale University, New Haven, CT
Hosted by: Hunter Peckham, Ph.D. Donnell Professor of Biomedical Engineering and Orthopaedics Case Western Reserve University Director, Functional Electrical Stimulation Center Louis Stokes Veterans Affairs Medical Center
MetroHealth Medical Center Abstract:
It is estimated that there are 250,000 people in the U.S. with spinal cord injuries (SCI) and over 2 million worldwide. While, the majority of SCI research has focused on regenerating neural tissue, recovery is correlated with angiogenesis. Furthermore, vessels
appear to play an important role in the neural stem cell (NSC) niche
and may promote the proliferation, and neuronal differentiation of
NSCs.
However, inducing angiogenesis following SCI has been challenging
since many of the factors and drugs permeabilize the surrounding
vessels and can exacerbate injury. Engineering vascular networks
could be an alternative to inducing extensive angiogenesis, but it has
been challenging to engineer vascular networks that are stable for long
times.
We sought to determine whether stable, microvascular networks
could be engineered using a coculture of primary ECs and NSCs. We
isolated primary rat NSCs and primary ECs and cocultured them in a
macroporous hydrogel based on poly(l-lysine) (PLL) and
poly(ethylene glycol) (PEG). Coculture of primary NPCs and ECs led to
stable, functional vessels up to 6 weeks with no signs of clot
formation in a subcutaneous model. Moreover, this coculture promoted
the formation of microvascular networks in a spinal cord model.
The coculture also led to the formation of the blood-spinal cord
barrier whereas the controls did not. This lays the groundwork for a
new approach to promote recovery and Regeneration following injury in
the CNS and may provide a new paradigm to understand and treat
neurological diseases and disorders in the CNS more broadly.
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